Location: Fung Auditorium
Principles and Applications of Cell Lysis and Filtration
Nekeisha Nogal, Ph.D.
University of California, San Diego
Abstract: Cell lysis, the process by which cells are disrupted to release intracellular contents, is one technique widely and routinely used for the separation of various biomolecules in the bioprocess industry. The choice of procedure is highly dependent on the nature of the product and the nature of the cell or tissue. This lecture will briefly present the fundamental concepts and techniques involved in Cell Lysis. We will then focus on the mechanical and chemical methods to remove intracellularly synthesized products. The primary learning objective of this lecture is to provide students with an advanced background in cell lysis-an upstream bioprocess unit operation often employed in biotechnology purification platforms. We will contrast this process with a contemporary case study that examines upstream options for production of monoclonal antibodies, where cell lysis is usually not desired.
Biosketch: Nekeisha Nogal is a postdoctoral fellow in the Department of Bioengineering at the University of California, San Diego. She holds B.S and M.S. degrees in Chemical Engineering and a Ph.D. in Biomedical Engineering. Her current research investigates the influence of extracellular matrix stiffness on maturation of cardiac myocytes and cardiac stem cells. As a recipient of the NIH-funded, Institutional Research and Career Development Award (IRACDA), her research training at UCSD is coupled with a mentored teaching experience at San Diego State University in the undergraduate general biology course. She has presented freshman seminar lectures at UCSD in BE87: Engineering the Heart. She has also lectured in BENG 161: Biochemical Engineering-Bioseparations and BENG 109: Engineering Statics and Dynamics.